Nitrate Standard Sodium Nitrate at 2,000 µM in a special stabilizing solution. SUPPLIED COMPONENTS Clear 96 Half Area Well Plates See for plate dimension data. Prostaglandin E2 Multi-Format Enzyme Immunoassay Kits This kit uses Nitrate and Nitrite Standard solutions calibrated to the US National Institute for Science and Technology Standard Reference Materials and ISO/IEC standards.Ĭortisol Enzyme Immunoassay Kits (Strip Wells)Ĭortisol Enzyme Immunoassay Kits (Whole Plate) The concentration of Nitrate in the sample is calculated by subtracting the measured Nitrite concentration from the Total Nitric Oxide concentration for the sample. The colored product is read and calculated as with the Nitrite determination above. After a 20 minute incubation at room temperature, Color Reagents A and B are added and incubated at room temperature for 5 minutes.
The reductase in combination with NADH reduces Nitrate to Nitrite. Total Nitric Oxide content is measured after the sample is incubated with Nitrate Reductase and NADH.
#DETECTX CORTICOSTERONE ENZYME IMMUNOASSAY KIT PDF SOFTWARE#
The concentration of Nitrite in the sample is calculated, after making a suitable correction for any dilution of the sample, using software available with most plate readers. The colored product is read at 550 – 570 nm. For Nitrite detection, samples are mixed with Color Reagents A and B and incubated at room temperature for 5 minutes. Both Nitrate and Nitrite standards are provided to generate standard curves for the assay and all samples should be read off the appropriate standard curve. Please read the complete kit insert before performing this assay. Nitric Oxide content is derived from the sum of Nitrate (-NO3) and Nitrite (-NO2). 43(4):553-556.ĪSSAY PRINCIPLE The DetectX Nitric Oxide Detection Kit is designed to quantitatively measure Nitrate and Nitrite present in a variety of samples. (1997) “Nitric Oxide Determinations: Much Ado About NO.-Thing?.” Clin.Chem. (2002) “Nitric Oxide Levels and Lipid Peroxidation in Plasma of Patients with Gastric Cancer.” Jpn. (2003) “Changes in Nitric Oxide Levels and Antioxidant Enzyme Activities May have a Role in the Pathophysiological Mechanisms Involved in Autism.” Clin. (2007) “Nitric Oxide Levels in Saliva Increase with Severity of Chronic Periodontitis.” J. (2010) “Oxidative/Nitrosative Stresses Trigger Type I Diabetes: Preventable in Streptozotocin Rant and Detectable in Human Disease.” Ann. Knott, AB and Bossy-Wetzel, E., (2010) “Impact of Nitric Oxide on Metabolism in Health and Agerelated Disease.”. (2007) “Nitric Oxide and Mitochondrial Signaling: From Physiology to Pathophysiology.” ATVB 27:2524-2531. Biol., 15:1-8.Įursalimsky, JD and Moncada, S. Seth, D and Stamler, JS., (2007) “The SNO-proteome: Causation and Classifications.” Curr. (2010) “Inorganic Nitrate and the Cardiovascular System”. (1991) “Endogenous Nitric Oxide: Physiology, Pathology and Clinical Relevance.” Eur. However, colorimetric methods can be applied to measure its stable break-down products, nitrate (-NO3) and nitrite (-NO2)10. The physical properties of Nitric Oxide make it challenging for direct detection methods. NO is linked not only to coronary heart disease, endothelial dysfunctions, erectile dysfunction, and neurological disorders, but also diabetes, chronic periodontitis, autism, cancer, and assorted age-related diseases5-9. The balance and dynamics of the regulatory/damage facets of NO are major forces in mitochondrial signaling and dysfunction4. Nitrosaylation is also a regulated post-translational modification in cell signaling3. It also allows NO to be easily converted to a toxic radical that can produce nitrosative damage to cells, organelles and molecules such as DNA. The reactive nature of nitric oxide allows it to act as a cytotoxic factor when released during an immune response by cells such as macrophages. It can be endogenously generated by constitutive or induced enzymes like Nitric Oxide Synthase or it can be orally ingested as nitrates and nitrites for rapid uptake into circulation and subsequent conversion2.
Acting through soluble guanylate cyclase activation, NO is an important physiological regulator of the cardiovascular, nervous, and immunological systems1. Validation Data Sensitivity, Linearity, etc.īACKGROUND Nitric oxide (NO) is a diffusible, transient, reactive molecule that has physiological effects in the picomolar-to-micromolar range. TABLE OF CONTENTS Background 3 Assay Principle 4 Related Products 4 Supplied Components 5 Storage Instructions 5 Other Materials Required 6 Precautions 6 Sample Types 7 Sample Preparation 7 Reagent Preparation 7-8 Assay Protocol 9 Calculation of Results 10 Typical Data 11-12 Please read this insert completely prior to using the product. Sample Types Validated: Water, Buffers, Serum, Plasma, Urine, Saliva, and TCM
NITRIC OXIDE Colorimetric Detection Kit 2 Plate Kit Catalog Number K023-H1
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